PCR kit «ROSSAmed ROSSAmed HLA B-27»
Reagent Description
The kit is designed to detect allele 27 of the human major histocompatibility complex locus B (HLA-B27) using polymerase chain reaction in real time. Whole peripheral blood and/or dried blood spots can be used as a biomaterial.
Specifications
- Storage conditions : -16…-24°С
- Expiration date : 12 mounth
- Number of studies : 100
Detailed description
To increase the efficiency of the reaction and reduce the risk of the formation of nonspecific products before the start of the reaction, the reagent kit provides a “hot” start.
The kit contains a protection system that allows you to avoid contamination of the PCR mixture with amplicons (products of previous reactions) and obtaining false positive results, through the use of the enzyme uracil-DNA glycosylase (UDG) and deoxyuridine triphosphate (dUTP).
The use of simple and identical protocols significantly reduces the risk of operator error at all stages of work.
Using a simple and fast amplification protocol, which allows you to obtain reliable research results in 80 minutes, which significantly increases the flow of laboratory tests.
The presence of endogenous control is recorded via the Hex fluorescence channel. The use of internal control allows not only to control all stages of the PCR study for each sample, but also to assess the adequacy of the collection of biomaterial and its storage.
A simple interpretation of the results: the presence of a fluorescent signal through the Fam channel registers the presence of the HLA-B27 alley, both in a homozygous and heterozygous state, in a biological sample.
Possibility of using various types of amplifiers with detection of results in “real time” mode. For example: tablet type – “DTprime”, “DTlight”, “DT-96” (DNK-Technology LLC, Russia), or CFX96 (Bio-Rad Laboratories, USA), qTOWER 3 (Analytik Jena, Germany), Gentier 96E/ 96T/96R (Tianlong, China), rotary-type detecting amplifier, for example, Rotor-Gene 6000, Rotor-Gene Q (QIAGEN, Germany) and similar devices with the appropriate technical parameters.