Reagent kits for detection – RNA SARS – CoV – 2 “ROSSAmed COVID-19 RT-PCR” and “ROSSAmed EXPRESS COVID 19 RT-PCR”

In connection with the emergence of new strains of SARS – CoV – 2, the sensitivity of the currently existing kits of reagents to new variants is an urgent issue. The variability of a virus is related to its evolution. According to WHO SARS-CoV-2 variant tracking data, new coronavirus variants are characterized by the presence of multiple mutations in the Spike gene.

Reagent kits designed to detect – RNA SARS – CoV – 2 “ROSSAmed COVID-19 RT-PCR” and “ROSSAmed EXPRESS COVID 19 RT-PCR” by RT-PCR with real-time detection of results and a set of reagents “ROSSAmed COVID -19 ISOTHERM” based on the method of colorimetric, isothermal, loop amplification contains primers and fluorescent probes specific for fragments of the ORFlab, E and N genes of the SARS CoV – 2 coronavirus genome according to the reference sequence NC_045512.2.

Enzymes of the SARS-CoV-2 genes, analyzed by ROSSA reagent kits, do not affect areas prone to mutations of Alpha, Beta, Gamma, Delta, Mu, Omicron, etc. strains.

Thus, the presence of mutations in different variants of the SARS-CoV-2 virus does not affect the detection of the COVID-19 virus by ROSSA reagent kits.