PCR kit «ROSSAmed HBV-DNA-quant»
Reagent Description
(Русский) Набор реагентов предназначен для количественного выявления ДНК вируса гепатита В, методом ПЦР с детекцией результатов в режиме «реального времени». Материалом для проведения ПЦР служат пробы ДНК, выделенные из плазмы/сыворотки.
Specifications
- Storage conditions : -16…-24°С
- Expiration date : 12 mounth
- Number of studies : 100
Detailed description
The kit is intended for the quantitative detection of hepatitis B virus DNA using the PCR method with detection of results in real time. The material for PCR is DNA samples isolated from plasma/serum.
– has a high sensitivity of 100 copies of hepatitis B DNA in 1 ml of sample
– high specificity over 98%
– the kit has been tested on the international panel for quality control of reagent kits “HBV DNA Evaluation Panel 01” Qnostics, Block 1 Todd Campus, West of Scotland Science Park, Glasgow, G20 0XA, Scotland, UK
– calibrators (PKO-1 and PKO-2) are included in the NA extraction stage, which allows you to control the efficiency of the isolation stage and more accurately calculate the amount of virus in the sample
– no risk of false positive results due to the presence of dUTP and the UDG enzyme in the reagents
– low risk of cross-contamination due to fewer operations
– ease of use of the kits in work
– adaptation to the main amplifiers – DT, DNA Technology; qTOWER³, Analytik Jena; CFX96, Bio-Rad; Rotor-Gene, Qiagen, etc.
– average analysis time taking into account the extraction stage: 150 minutes
– number of reactions – 100